OLIS RSM 1000用于雜化細(xì)菌視紫紅質(zhì)/量子點(diǎn)體系的可調(diào)諧光循環(huán)動(dòng)力學(xué)研究
Tunable photocycle kinetics of a hybrid bacteriorhodopsin/quantum dot system

在生物環(huán)境中包含無(wú)機(jī)納米粒子已導(dǎo)致產(chǎn)生用于各種應(yīng)用的混合納米系統(tǒng)。一種這樣的系統(tǒng)包括與光活性蛋白質(zhì)細(xì)菌視紫紅質(zhì)（BR）偶聯(lián)的量子點(diǎn)（QD），其已經(jīng)在開(kāi)發(fā)增強(qiáng)型光伏器件中進(jìn)行了探索。在這項(xiàng)工作中，我們發(fā)現(xiàn)可以使用CdSe / CdS（核/殼）QD來(lái)控制BR光循環(huán)的動(dòng)力學(xué)。使用時(shí)間分辨吸收光譜法監(jiān)測(cè)具有不同QD量的蛋白質(zhì)樣品的光循環(huán)壽命。在動(dòng)力學(xué)跡線中觀察到bR和M狀態(tài)壽命的濃度依賴性伸長(zhǎng)，因此表明BR和QD之間發(fā)生激子耦合。我們提出BR與QD的配對(duì)有可能用于基于蛋白質(zhì)的計(jì)算應(yīng)用，特別是用于實(shí)時(shí)全息處理器，其依賴于bR和M光中間體的時(shí)間動(dòng)態(tài)。

The inclusion of inorganic nanoparticles in biological environments has led to the creation of hybrid nanosystems that are employed in a variety of applications. One such system includes quantum dots (QDs) coupled with the photoactive protein, bacteriorhodopsin (BR), which has been explored in developing enhanced photovoltaic devices. In this work, we have discovered that the kinetics of the BR photocycle can be manipulated using CdSe/CdS (core/shell) QDs. The photocycle lifetime of protein samples with varying QD amounts were monitored using time-resolved absorption spectroscopy. Concentration-dependent elongations of the bR and M state lifetimes were observed in the kinetic traces, thus suggesting that excitonic coupling occurs between BR and QDs. We propose that the pairing of BR with QDs has the potential to be utilized in protein-based computing applications, specifically for real-time holographic processors, which depend on the temporal dynamics of the bR and M photointermediates.

https://link.springer.com/article/10.1007/s12274-018-2224-4